Which of the following actions does NOT demonstrate reduction in animal use?

The action that does not demonstrate a reduction in animal use is implementing a new anesthetic that improves recovery. This choice focuses on enhancing the welfare of the animals during and after procedures rather than actively reducing the number of animals used in research. While improving recovery is certainly beneficial and can lead to better overall animal health and well-being, it does not directly reduce the amount or number of animals used in studies.

Reduction in animal use, a key principle of the 3Rs (Replacement, Reduction, Refinement), specifically refers to designing studies and experiments in a way that minimizes the number of animals required to achieve scientifically valid results. Using less invasive procedures or lowering the frequency of interventions focuses on maintaining animal numbers while possibly improving their welfare. Conducting more studies with fewer animals emphasizes efficiency in research design, which directly contributes to minimizing overall animal use.